During the scene where Annie is chasing after Stass her sensible running shoes are clearly visible, but when she is slumped over in the subway station, she is back in very high heels.
(at around 42 mins) After they cross the train tracks during the car chase, both drivers are seated on the right.
When Annie walks into her sister's kitchen on the second morning, when she's wearing the bright blue blouse, her jacket is buttoned. When she high fives her niece the view switches and her jacket is now unbuttoned, then the view switches back and her coat is buttoned again, and then the view switches to one from behind her sister and the jacket is again unbuttoned.
When she says, "DEC method; determine, evade, coerce," during the car chase, the background behind the car can be seen to jump.
When Stass is preparing to assassinate Petrov he lays his gun on a serving cart under a staggered stack of 5 or 6 folded cloth napkins. However, when he reaches his destination the gun is now inside a single folded napkin laying on top of all the other napkins.
The replication of the DC Metro was not even close. The stations and trains do not come close to those of the real DC Metro Station. The term subway is not used, it is called the Metro.
After Annie first meets Augie and she walks away, he says he loves kitten heels. Kitten heels are about 1" high. She is wearing about 4" pumps.
When Annie lands from her parachute jump, there is a pole with a windsock in the middle of the landing zone. This would be very dangerous to anyone who actually landed in the center of the target. Windsocks would normally be located several hundred feet away from a jump zone to prevent injuries.
When Annie first gets into the Langley building, after sky diving at the farm, they take her picture and record her fingerprints. They also get a picture of her eyes, possibly for reference, or security reasons. The picture of the eye is a hazel eye, with some light brown and green. Annie's (Piper Perabo's) eyes are actually a very dark brown.
The CIA gives a lifestyle polygraph prior and during employment. Many of the polygraph questions were job interview related and her relationship. Most likely they would delve deeper in her foreign travel and contacts that she had made especially any foreign national contacts.
When Arthur Campbell receives a fax, the printed fax can clearly be seen going into the machine as well as out and the copy button is lit.
Annie is involved in a car chase, during which she follows a car under a freeway. During the sequence she has to mount the sidewalk in her car. A series of planks of wood, painted black, are arranger on the road up to the edge of the curb to allow the car to mount the pavement. Without them the cars limited clearance would mean that it wouldn't have otherwise managed it.
When Auggie checks his braille watch, he reads it like the tactile type by flipping the crystal up, but we hear the time announced as if it were the talking watch type. Generally speaking, blind people use one mode or the other, but not both at the same time. It would be redundant, and hearing the time announced is not always wanted.
In car chase sequence, as Anne is following the car and goes through the ally, you can see her driver side taillights is white for just that one angle and the goes back to red after.
There are just so many errors with allegedly Washington DC buildings and geography! But the most egregious is the metro station marked "Subway." In DC it is the Metro, not the subway, and nowhere in the system is there a sign marked "Subway."
During the skydiving scene, the instructor notes that they are only 2,000 feet AGL (above ground level), but Annie is in freefall for about 30 seconds and she would have hit the ground in about 15 seconds, 20 seconds maximum. The aerial views shown are also clearly from an altitude of much higher than 2,000 feet.