Opening sequences include depictions of Monument Valley, which featured in many of Director John Ford's westerns.
At the end of the cartoon, Daffy is pushing a street-sweeper's cart with "D.S.C." emblazoned on the side. This stands for the "Department of Street Cleaning", which was created in New York City in 1881 and renamed the Department of Sanitation in 1929. Daffy also is wearing a cartoon adaptation of that department's original medical-white uniform with white police-type helmet.
Daffy Duck says, "Hi-Yo, Tin Foil!" before riding into town. This is a play on the Lone Ranger, who would say, "Hi-Yo, Silver!" to get his horse, Silver, to run off towards a town or chase a bad guy.
The wanted poster for Nasty Canasta, offering a $5 million reward for his dead body (not "wanted dead or alive"; just "dead"), lists his crimes as "rustler, bandit, square dance caller". This is an inside joke, as square dancing was a frequent pastime of the employees in the Warner Brothers animation department in the 1950s, most notably director Chuck Jones.
The mountains depicted in the opening scenes are all thin, a comment on western movies where town buildings are really only the front with nothing on the other side of the building.