This film was rarely seen for many years after its original release in 1965. As a result of this, it was the only one of the original "Fly" films that never received a VHS or LaserDisc release. It did not even receive a home video premiere at all until 2007, when it was released on DVD for the first time ever, in "The Fly Collection", a 4-disc box set that contained both it and the previous two films in the trilogy, The Fly (1958) and Return of the Fly (1959), as well as a special features DVD.
Vincent Price's character of Francois Delambre does not appear in this film, the third and final one of the original "Fly" trilogy. By the time it was produced, Price was already under contract to American International Pictures.
Many fans of The Fly (1958) and Return of the Fly (1959), say that this film has no connection at all with the previous two films, mainly because no Fly monster is shown, but with the central character having the last name Delambre, a photograph showing the Fly appearing during a conversation about the Delambre family legacy, a new version of the teleporter and a scene with Inspector Charas (played by a different actor this time), this film definitely has connections to both The Fly (1958) and Return of the Fly (1959). Produced cheaply in England, it was a box office bomb because audiences wanted to see a man with a fly's head, and they did not get to see this at all. This was the last of "The Fly" films released by 20th Century Fox for many years, until the 1986 remake with Jeff Goldblum and Geena Davis.