The title of the episode is a parody of the musical Annie Get Your Gun.
This episode marks the first in a series of recurring jokes on the show, in which Stan calls the CIA lab, requesting news on an outlandish experiment and them replying "uh, we gotta call you back".
Carnival music later re-used in "The Cleveland Show" {Flush of Genius (#3.20)}.
The National Gun Association is a parody of the real-life National Rifle Association (NRA).