Lola Lasagne (Ethel Merman) mentions her three-week marriage to Luigi Lasagne. This is clearly Merman being a good sport; the joke refers to her very brief marriage to Ernest Borgnine three years earlier.
All the horse race scenes were filmed in one day, on July 11, 1967 at Los Alamitos Race Track.
Batman recites some of the lyrics of the "Batgirl" theme as he ponders her identity.
Horse racing is known as The Sport of Kings, thus the title.
The Bat Shield, though campy, is modeled after plexiglass shields used by first responders and the military.