Stasia Masters, pumping Barry Allen for information on the Flash's origins, speculates that he was "built" by some mad scientist, whom Barry calls "Professor Zoom". Professor Zoom (a/k/a The Reverse Flash) was one of the Silver Age Flash's classic villains in the DC Comics series. Also, this episode features another reference to "The Hotel Infantino", a reference to Silver Age Flash co-creator Carmine Infantino.
David Cassidy's daughter Katie Cassidy would later play Laurel Lance / Black Canary in Who Is Harrison Wells? (2015) and her Earth Two counterpart Laurel Lance / Black Siren in Invincible (2016).
Garrick Gallery, the art gallery visited by Barry and Tina, is a reference to Jay Garrick, the Golden Age Flash.
The first three digits of Barry's Social Security Number are 542, indicating he was born in Oregon.
At around 13 minutes there is a beaker labeled with only a Bat Symbol. Possible Batman reference.