If the player encounters the Green Toad in any galaxy where he's digging with a pickax, zooms in his face and shoots him with a Star Bit, his facial expression changes from happy to sad. He will remain sad until the galaxy is complete, or if the player loses a life. He is the only Toad who does this.
First appearance of Rosalina. She has since been a recurring character in Mario games.
The Star Bits that the Lumas love to eat are based off of a type of Japanese candy called "konpeitô".
Rosalina is known as "Rosetta" in the Japanese version, and the Lumas are called "Chiko".
The first time in the Mario series to utilize orchestral performances for soundtracks in-game.