A rival group of snakehunters are creeping into Dusty's area. So, the team decide to go hunting at night time, which is much for dangerous than in the day.
Dusty leads the team to an abandoned missile factory near the epicenter of the snake epidemic that could hold the biggest concentration of pythons in the Everglades.
Dusty and his team find a frightening new hybrid Python species when they trek deep into the dense Python Pines to prevent the snakes from laying their eggs.
When a potentially record-breaking python escapes Dusty's grasp, his obsession to catch it brings him face to face with the massive reptile in the most dangerous solo mission of his career.