Botanica is the only plant-based Transformer ever to have existed. Besides that, she is the only Transformer to have two differently colored eyes.
No toy of Botanica was ever produced, even though she is a major character in the series.
Thrust clearly, albeit very quickly flips off Optimus Primal with his middle finger, to which he responds with an annoyed and displeased face.
The end of this episode is the only time Megatron's big floating head transforms. Strangely, it seems to bear a resemblance to Megatron's earlier Predacon ship, the Darksyde.
Silverbols and Nightscream make a reference to the popular board game Monopoly as they say, rather randomly, "Do not pass 'Go!'" "Do not collect $200!".