This is Dr. Simon's first appearance. Dr. Simon was a remake African-American version of Dr. Weston (voiced by Victoria Carroll), a veterinarian from the Pound Puppies TV Special. She is not related to the Simon Family from Snowbound Pound.
This is the first time when Season 2 was also known as "The All-New Pound Puppies".
Nose Marie now wears a dress with an apron and sports a kind and loving personality, making her a surrogate mother and older sister to the younger puppies.
Bright Eyes is redesigned as an even younger puppy than she was in Season 1. She also wears a blue shirt and a blue skirt instead of green.
Holly is redesigned to be older than she was in Season 1. She now has longer hair and wears jeans. She no longer wears her trademark scarf. In Season 1, she is nine years old. In Season 2, she is eleven years old, meaning that she is now only six months younger than Brattina. This may hint that Season 2 takes place two years after the events in Season 1.