In the segment "Pinta Island Tortoise" Lonely Hubert is an obvious reference to the famous Pinta Island Tortoise, Lonesome George. In a sad coincidence Lonesome George, the last of his species, died on June 24, 2012, he was 101 years old. The segment had already been filmed but aired roughly two months after George's demise. Although the species is officially extinct there are some very close cousins alive, in 2007 researchers from Yale found 17 first-generation tortoise hybrids that have Pinta tortoise DNA, indicating there is possible a pure bred Pinta still alive on the island. In 2013 the researchers returned and began DNA testing the tortoises on the island, as of 2020 they have not yet found a pure Pinta but they did find a female hybrid that is a direct descendant of George's ancestors.
The voice of "The Narrator" parodies Morgan Freeman in "March of the Penguins" (2005).
At one point, Zoidberg (as a lobster) says: "I'll try a bite". This sounds like "I'm trilobite". Trilobites are an ancient, extinct subgroup of arthropods. Crustaceans (including Zoidberg) are a subgroup of arthropods as well.