On the jet Coulson tells Ward if anything goes wrong, he'll spend the rest of his career in Alaska doing night shift on Blonsky's cryo cell. Blonsky refers to Emil Blonsky, AKA the Abomination, the main enemy from The Incredible Hulk (2008).
Ming-Na Wen suffers from a fear of heights and had to muster up all her courage to film the scene of May walking atop the moving train.
Agent Coulson (Clark Gregg) reveals that The Fridge is located in Barrow, Alaska. This is the northernmost city in the United States, located on the north coast of the state, and experiences two months of darkness in the winter.
Because there was no equipment in existence that would fit to stand in for the hyperbaric chamber Mike was kept in, the production team had to design and build one themselves, and they had to do it quickly. So much so, that the paint was still literally drying when the set was wheeled in for filming.
At 40:05- Hollywood cliche #089: male actors must always punch something when they are angry.