When the Pizza Cats and their main enemies are in at the time machine, Polly is to Speedy's right and Guido to Speedy's left. Then in a shot of Guido asking what's happening along with Speedy and Polly commenting what's going on, Guido and Polly have switched positions. Then in the POV shot of everyone before the time travel, Polly and Guido are back in their original positions.
When Bad Bird blames the Pizza Cats for screwing up the time machine, Big Cheese's snout and nose is the same color as his face.
When Polly is stalking Big Cheese (15 years ago) from in a tree and throws a big heart at him, the visor on top of her helmet is dark red instead of pink.