Billy Bryk's (Billy Clanton) real-life father, Greg Bryk previously played Jack of Knives in the show.
When Wynonna's hood is taken off, Deputy Assistant Director Naomi Hycha says to dim the lights because they aren't at the dentist. In real life, Doc Holliday was a dentist.
Both Melanie Scrofano (Wynonna Earp) and Reagan Pasternak (Deputy Assistant Director Naomi Hycha) have appeared on the TV show, Being Erica (2009), playing Rebecca and Julianne Giacomelli respectively.